The method was successfully applied for determination of both drugs in pharmaceutical dosage forms and real human plasma. The calibration plots displayed good linear relationships in the concentration ranges of 0.5–50 and 0.75–75 μg/mL for norfloxacin and tinidazole, respectively. Tinidazole and norfloxacin were eluted with retention times of 1.8 and 5.8 min, respectively. The mobile phase was delivered isocratically at a flow rate of 1 mL/min with UV detection at 290 nm. The optimal mobile phase composition was predicted to be: 3.5% w/v SDS, 10.03% v/v 1-propanol, 0.5% v/v 1-octanol and 0.3% triethylamine in 0.02 M phosphoric acid at pH 6.5. While the location of optimum conditions was established by applying Derringer's desirability function. A 27-4 fractional factorial design was used as screening design. Optimization experiments were conducted through a process of screening and optimization. The aim of the present study was to optimize a microemulsion liquid chromatography method for the simultaneous determination of norfloxacin and tinidazole binary mixture using a chemometric protocol. The newly synthesized copper complexes showed overall better biological activities compared to each parent ligands used. S-1 and S-5 were found to be most inhibitors of α-amylase and α-glucosidase having IC50 42.50, 44.80 and 4.52 µg/mL, 4.80 µg/mL, respectively. Significant α-amylase and α-glucosidase inhibition activities were shown by the synthesized complexes. The antibacterial potential and α-amylase and α-glucosidase inhibition studies of complexes were further investigated by molecular docking studies, which supported the experimental results.
α-Amylase and α-glucosidase inhibition studies of the synthesized complexes were also carried out. Agar diffusion method was employed to investigate in vitro antibacterial activities of the synthesized metal complexes and the tested parent ligands. Different bacterial and fungus strains were ascertained to evaluate the biological potency of the synthesized complexes, that is, Escherichia coli, Bordetella bronceptica, Staphylococcus epidermidis, Baccilus pumilus, Staphylococcus aureus and yeast strain Saccharomyces cerevisiae. Synthesis and characterization of novel copper complexes of metronidazole benzoate (MTZ Benz), metronidazole (MTZ) in the presence of another ligand dichloroacetic acid (DCA) were compared and reported in the present work. The developed method could separate the mixture as following amoxicillin and omeprazole at 2.56, 4.84, respectively, where metronidazole and clarithromycin were retained 3.04 min. Regarding the optimum amount of trimethylamine added to the mobile phase to improve the resolution, the outcomes showed that 30 µL was the best choice at pH around 6.0 with 0.05 M potassium dihydrogen phosphate as a buffer. Moreover, better resolution was achieved at mobile phase compositions of 30:30:40 (acetonitrile: methanol: buffer), respectively. The results showed that amoxicillin was detected at 254 nm, where metronidazole, omeprazole and clarithromycin were detected at 304 nm. Design of experiments was then implemented to evaluate the best separation parameters. The first result showed that C18 reversed phase column has better resolution than C8 for analyzing amoxicillin, metronidazole, omeprazole and clarithromycin at isocratic elution mode. In this study, RP-HPLC method was developed in terms of mobile phase composition, buffer concentration and additive amount to separate the complex drugs mixture used as triple therapy for H. Because of the resistance of this organism, triple therapy treatment is required. pylori) is the most chronic bacterial infection on human being that is found in the gastric mucous layer and adapted to survive in acidic conditions.
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